Please use this identifier to cite or link to this item: http://hdl.handle.net/2307/323
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dc.contributor.authorDettin, Monica-
dc.contributor.authorThushari, Herat-
dc.contributor.authorGambaretto, Roberta-
dc.contributor.authorIucci, Giovanna-
dc.contributor.authorBattocchio, Chiara-
dc.contributor.authorBagno, Andrea-
dc.contributor.authorGhezzo, Francesca-
dc.contributor.authorDi Bello, Carlo-
dc.contributor.authorPolzonetti, Giovanni-
dc.contributor.authorDi Silvio, Lucy-
dc.date.accessioned2010-07-15T08:52:15Z-
dc.date.available2010-07-15T08:52:15Z-
dc.date.issued2009-11-
dc.identifier.urihttp://hdl.handle.net/2307/323-
dc.description.abstractBioactive molecules have been proposed to promote beneficial interactions at bone-implant interfaces for enhancing integration. The main objective of this study was to develop novel methods to functionalize oxidized titanium surfaces by the covalent immobilization of bioactive peptides, through selective reaction involving single functional groups. In the first protocol, an aminoalkylsilane was covalently linked to the Ti oxide layer, followed by covalent binding of glutaric anhydride to the free NH2 groups. The carboxylic group Of glutaric anhydride was used to condense the free N-terminal group of the side-chain protected peptide sequence. Finally, the Surface was treated with trifluoroacetic acid to deprotect side-chain groups. In the second protocol, the peptide was directly anchored to the Ti oxide surface via UV activation of an arylazide peptide analogue. X-ray photoelectron spectroscopy analyses confirmed that modifications induced onto surface composition were in agreement with the reactions performed. The peptide density of each biomimetic Surface was determined on the basis of radiolabeling and XPS derived reaction yields. The in vitro cellular response of the biomimetic surfaces was evaluated using a primary human osteoblast cell model. Cell adhesion, proliferation, differentiation, and mineralization were examined at initial-, short-, and long-time periods. In was shown that the biomimetic surface obtained through photoprobe-marked analogue that combines an easily-performed modification provides a favorable surface for an enhanced cellular response. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 463-479, 200en
dc.language.isoenen
dc.publisherWiley-Lissen
dc.relation.ispartofJournal of Biomedical Materials Research Aen
dc.subjectbiomimetic surfaceen
dc.subjectcell adhesionen
dc.subjecttitanium oxideen
dc.subjectpeptideen
dc.subjectXPSen
dc.titleAssessment of novel chemical strategies for covalent attachment of adhesive peptides to rough titanium surfaces: XPS analysis and biological evaluationen
dc.typeArticleen
dc.subject.miurSettori Disciplinari MIUR::Scienze chimicheen
dc.subject.isicruiCategorie ISI-CRUI::Scienze chimicheen
dc.subject.anagraferoma3Scienze chimicheen
dc.relation.volumenumber91Aen
dc.relation.pagenumber463-479en
dc.identifier.doi10.1002/jbn.a.32222-
dc.relation.issuenumber2en
item.languageiso639-1other-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
Appears in Collections:A - Articolo su rivista
X_Dipartimento di Fisica 'Edoardo Amaldi'
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